Title: Isolation, Screening and Optimization of Xylanase Production in Submerged Fermentation Using P.citrinum.
Authors: Ghoshal, G; Banerjee, U C; Shivhare, U S
Abstract: Cellulase free xylanase is one of the most
important upcoming enzyme having industrial application in chemical,
biochemical, pharmaceutical and food industry. In the present study, a soil
isolate was found to be promising for xylanase production. Biochemical
identification tests, Scanning Electron Microscopy (SEM) and 16S rRNA
sequencing identified the organism Penicillium citrinum MTCC 9620. Later
on, optimization of media composition and environmental factors were
investigated. Temperature, pH, agitation speed, carbon sources, nitrogen
sources, inoculum concentration, and additives were optimized on the basis of
xylanase yield, dry cell weight, extracellular protein content, sugar
consumption etc. Optimized media produced 138.73 U/mL xylanase at 120 h (30°C).
The purified xylanase was found to have molecular weight approximately 29 KDa.
Page(s): 400-405
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